Class 12 Biology Practical Syllabus 2026, Download PDF

The Biology 12 Practical Syllabus 2026 contains one major experiment, one minor experiment, slide preparation, spotting, practical record, viva voce, investigatory project, and its record and viva voce. Students must choose one and prepare a report on it for submission. The CBSE Class 12 Biology Practical exam carries 30 marks and will be held from January 1st to January 14th, 2026, as per the CBSE datesheet. Scroll down the page to find the list of experiments, identification and more.

Class 12 Biology Practical Syllabus 2026

The Class 12 Biology practical exam 2026 is worth 30 marks. These students will have a major & a minor experiment of 5 marks each, a slide preparation of 5 marks. They will also get 7 marks for spotting.

Biology Practical Syllabus 2026 Class 12 Marking Scheme

Class 12 Biology Practical Syllabus 2026

Learn the list of experiments for the Class 12 Biology Practical Exam 2026 below –

Section A: List of Experiments

1. Prepare a temporary mount to observe pollen germination.
2. Study the plant population density by the quadrat method.
3. Study the plant population frequency by the quadrat method.
4. Prepare a temporary mount of the onion root tip to study mitosis.
5. Isolate DNA from available plant material such as spinach, green pea seeds, papaya, banana, etc.

Section B. Study and observe the following (Spotting)

1. Flowers adapted to pollination by different agencies (wind, insects, birds).
2. Pollen germination on stigma through a permanent slide or scanning electron micrograph.
3. Identification of stages of gamete development, i.e., T.S. of testis and T.S. of ovary through permanent slides (from grasshopper/mice).
4. Meiosis in onion bud cells or grasshopper testis through permanent slides.
5. T.S. of blastula through permanent slides (Mammalian).
6. Mendelian inheritance using seeds of different colours/sizes of any plant.
7. Prepared pedigree charts of any one of the genetic traits such as rolling of tongue, blood groups, ear lobes, widow’s peak and colour blindness.
8. Controlled pollination – emasculation, tagging and bagging.
9. Common disease-causing organisms like Ascaris, Entamoeba, Plasmodium, any fungus causing ringworm through permanent slides, models or virtual images or specimens. Comment on the symptoms of diseases that they cause.
10. Model specimens showing symbiotic association in lichens, root nodules of leguminous plants, and the parasitic mode of nutrition shown by Cuscuta on the host.
11. Flash cards/models showing examples of homologous and analogous organs.

A. Items for identification/familiarity with the apparatus for practical assessment

(All experiments) Beaker, flask, petriplates, soil from various areas – sandy, clayey, loamy, small potted plants, aluminium foil, paint brush, test tubes, starch solution, iodine, ice cubes, Bunsen burner/spirit lamp/water bath, huge flowers, Maize inflorescence, model of embryonic stages showing morula and blastula of frog, beads/seeds of various shapes, sizes, and textures Ascaris, cactus/opuntia (model).

B. List of Practicals

1. Study of flowers suited to pollination by various agents (wind, insects).
2. Identification of T.S. of morula or frog blastula (Model).
3. Study the Mendelian inheritance pattern with beads/seeds of various sizes/textures.
4. Creation of pedigree charts for genetic characteristics such as rolling the tongue and colour blindness.
5. Investigate emasculation, tagging, and bagging by conducting controlled pollination experiments.
6. Recognise common disease-causing organisms such as Ascaris (model) and study some of the signs of the diseases they cause.
7. Discuss the morphological adaptations of plants living in xerophytic environments.

Note: Instead of recording observations, the preceding practicals might be completed experientially.

Class 12 Biology Practical Syllabus 2026 PDF Download

The CBSE Class 12 Biology Practical Syllabus PDF for 2025-26 includes experiments and investigatory projects. Download the full PDF for a detailed experiment list and marking scheme below.

Class 12 Biology Practical Syllabus 2026 PDF